Toll-like receptor 5-mediated signaling enhances liver regeneration in mice

Abstract Background Toll-like receptor 5 (TLR5)-mediated pathways play critical roles in regulating the hepatic immune response and show hepatoprotective effects mouse models of diseases. However, role TLR5 experimental liver regeneration has not been reported. This study aimed to investigate partial hepatectomy (PHx)-induced regeneration. Methods We performed 2/3 PHx wild-type (WT) mice, knockout or agonist CBLB502 treated as a model Bacterial flagellin content was measured with ELISA, expression determined quantitative PCR analyses flow cytometry. To on hepatocyte proliferation, we analyzed bromodeoxyuridine (BrdU) incorporation proliferating cell nuclear antigen (PCNA) immunohistochemistry (IHC) staining. The during priming phase were examined immediate early gene mRNA levels, Western blotting analysis NF-κB STAT3 activation. Cytokine growth factor production after detected real-time cytometric bead array (CBA) assays. Oil Red O staining lipid concentrations examine effect accumulation PHx. Results bacterial serum increased, significantly up-regulated WT mice TLR5-deficient exhibited diminished numbers BrdU- PCNA-positive cells, suppressed expression, decreased cytokine production. Moreover, PHx-induced activation inhibited Tlr5 −/− compared mice. Consistently, administration promoted PHx-mediated which correlated enhanced proinflammatory cytokines recruitment macrophages neutrophils liver. Furthermore, displayed lower smaller positive areas than those control Conclusion reveal that contributes initial events Our findings demonstrate signaling positively regulates suggest potential promote